Construction Standards of Biological Aseptic Rooms
2024.12.27
In the era of the booming development of modern life sciences, biopharmaceuticals, and other cutting - edge fields, biological aseptic rooms, as the core places for conducting key experiments and production activities, have attracted much attention regarding their construction standards. Guangzhou Cleanroom Construction Co., Ltd., as a professional manufacturer of purification equipment with years of experience in the industry, will provide you with a detailed interpretation of the construction standards of biological aseptic rooms today.
I. Spatial Layout and Zoning Planning
The layout of a biological aseptic room is by no means random. It needs to be designed scientifically and rigorously according to the experimental or production process. Firstly, distinct functional areas should be demarcated, generally including a preparation area, a buffer area, an experimental operation area, and an auxiliary area. The preparation area is used for the pre - sorting and disinfection of experimental supplies. The buffer area, like a "transition bridge," effectively isolates the outside from the core operation area. Personnel change into clean work clothes and clean their hands here to reduce the risk of bringing in contamination. The experimental operation area is the core of the core, where all delicate operations such as biological sample processing and cell culture are carried out, and it has extremely high requirements for an aseptic environment. The auxiliary area is used to store equipment and reagents, ensuring the convenience of material supply. There should be a reasonable air flow direction among the various areas to avoid cross - contamination. Usually, the principle of a one - way air flow from low - risk areas to high - risk areas is followed.
II. Requirements for the Air Purification System
This is the "life - support system" of a biological aseptic room. The primary - efficiency filter is responsible for intercepting large particles in the air, such as dust and hair, preventing them from entering subsequent purification processes. The medium - efficiency filter further captures particles of 1 - 5μm. The high - efficiency filter, known as the "guardian," should have a filtration efficiency of over 99.99% for microbial aerosol particles of 0.3μm and smaller, ensuring that the air sent into the aseptic room is pure and sterile. At the same time, a stable and reliable air - conditioning system is equipped to precisely regulate the temperature between 22 - 26°C and maintain the relative humidity at 45% - 60%. This creates a suitable and stable temperature and humidity environment for biological experiments or production, inhibits the growth of microorganisms, and ensures the comfort of experimental samples and operators.
III. Selection of Enclosure Structure Materials and Sealing Performance
The materials selected for the walls, ceilings, and floors are directly related to the overall performance of the aseptic room. Color - coated steel plates are preferred for the walls. They have a smooth and flat surface, are not prone to dust accumulation, have good antibacterial properties, and are easy to clean and disinfect. The ceiling also uses color - coated steel plates with good sealing performance to ensure that no dust leaks. For the floor, anti - static, corrosion - resistant, and easy - to - clean epoxy self - leveling floor materials are given priority. They can prevent the adsorption of microorganisms caused by static electricity and can withstand the corrosion of chemical reagents. The entire enclosure structure must ensure extremely high sealing performance. Details such as door seams and corners are sealed with materials such as rubber seals to prevent unpurified air from the outside from sneaking in and disrupting the aseptic environment.
IV. Provision of Disinfection and Sterilization Facilities
A biological aseptic room requires comprehensive disinfection and sterilization methods. Ultraviolet disinfection lamps are common and basic equipment. They are distributed in every corner of the room. When there is no one in the room, they are turned on to damage the DNA structure of microorganisms through ultraviolet irradiation, achieving preliminary disinfection of the air and object surfaces. In addition, appropriate chemical disinfectant spraying devices should be equipped. Depending on different pollution situations, disinfectants such as hydrogen peroxide and alcohol are selected to regularly conduct in - depth disinfection of equipment, countertops, floors, etc. For some heat - resistant experimental utensils, dry - heat sterilizers or autoclaves can be set up to kill microorganisms using high temperatures, ensuring that every corner and every item in the aseptic room is in an aseptic state.
V. Management of Personnel and Material Entry and Exit
Personnel are one of the greatest potential sources of pollution in an aseptic room. Therefore, a strict personnel entry and exit process must be set up. Before entering, personnel need to change into special clean work clothes, hats, masks, and shoe covers in the changing room. After passing through the air shower to blow off the dust attached to their bodies, they can enter the buffer area and then gradually enter the core operation area. The entry and exit of materials should also not be overlooked. Special transfer windows are set up, and methods such as ultraviolet disinfection and self - cleaning functions are used to ensure that the materials are free of surface contamination before entering the aseptic room, preventing the invasion of foreign microorganisms.
The construction standards of biological aseptic rooms cover numerous details, and every link is closely interlocked to create a highly aseptic scientific research and production environment. Guangzhou Cleanroom Construction Co., Ltd., with its exquisite technology and rich experience, can customize high - standard biological aseptic room solutions for customers, helping the life science and biopharmaceutical industries take off. If you have any questions or needs regarding the construction of biological aseptic rooms, please feel free to contact us at any time!
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